Fluorescence Microscopy Visualization of Contacts Between Objects

Open Access
Authors
Publication date 16-03-2015
Journal Angewandte Chemie, International Edition
Volume | Issue number 54 | 12
Pages (from-to) 3688-3691
Number of pages 4
Organisations
  • Faculty of Science (FNWI) - Van 't Hoff Institute for Molecular Sciences (HIMS)
  • Faculty of Science (FNWI) - Institute of Physics (IoP)
Abstract The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz's classical theory based on elastic deformation.
Document type Article
Note With supplementary file
Language English
Related publication Fluorescence Microscopy Visualization of Contacts Between Objects
Published at https://doi.org/10.1002/anie.201410240 https://doi.org/10.1002/ange.201410240
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Fluorescence Microscopy (Final published version)
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