In vivo visualization and quantification of mitochondrial morphology in C. elegans

Authors
Publication date 2015
Host editors
  • V. Weissig
  • M. Edeas
Book title Mitochondrial medicine. - Vol. 2: Manipulating mitochondrial function
ISBN
  • 9781493922871
Series Methods in molecular biology, 1265
Pages (from-to) 367-377
Publisher New York: Humana Press
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.
Document type Chapter
Language English
Published at https://doi.org/10.1007/978-1-4939-2288-8_26
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