Search results
Results: 55
Number of items: 55
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Kramer, G. (2025). MassIVE MSV000099169 - Capturing the RNA-binding proteome from plants using orthogonal organic phase separationSummary RNA-binding proteins (RBPs) regulate many processes related to RNA biogenesis, localization, half-life and function. Here, we present a protocol for the en masse isolation of RBPs cross-linked to RNA and provide a strategy for proteomics analysis. We describe the steps for in vivo UV-crosslinking of RNA-protein complexes, tissue lysis, fractionation and purification of crosslinked RNA-protein adducts using organic solvents. Although the protocol was developed for Nicotiana benthamiana leaves, it can be optimized for use in different plants and tissues. [Data set]. MassIVE. https://doi.org/10.25345/c5kk94r4h
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Hansildaar, R., van Velzen, M., van der Vossen, E. W. J., Kramer, G., Nurmohamed, M. T., & Levels, J. H. M. (2025). Plasma proteome analysis of rheumatic patients reveals differences in fingerprints based on cardiovascular history: a pilot study. Proteome Science, 23, Article 4. https://doi.org/10.1186/s12953-025-00243-6 -
Koopman, N., Jaspers, Y., van Leeuwen, P. T., Chronas, K., Li Yim, A. Y. F., Diederen, K., te Velde, A. A., Roseboom, W., Kindermann, A., Benninga, M. A., Kramer, G., de Jonge, W. J., Brul, S., Levin, E., Kemp, S., & Seppen, J. (2025). Integrated multi-omics of feces, plasma and urine can describe and differentiate pediatric active Crohn's Disease from remission. Communications medicine, 5, Article 281. https://doi.org/10.1038/s43856-025-00984-7 -
Tu, Z., Li, Y., Ji, S., Wang, S., Zhou, R., Kramer, G., Cui, Y., & Xie, F. (2025). Gas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis. Heliyon, 11(2), Article e41928. https://doi.org/10.1016/j.heliyon.2025.e41928 -
Pasman, R., Krom, B. P., Jonker, M. J., de Leeuw, W. C., Kramer, G., Brul, S., Zaat, S. A. J., & Zhang, J. (2025). Candida albicans and Staphylococcus aureus reciprocally promote their virulence factor secretion and pro-inflammatory effects. Frontiers in Cellular and Infection Microbiology, 15, Article 1629373. https://doi.org/10.3389/fcimb.2025.1629373 -
Dangol, S., Alves Leite, M. F., Neli Alves, M., Galland, M., van Kleeff, P. J. M., Kramer, G., Darolt, J. C., Raiol-Junior, L. L., Brandão Garcia, R., Peña, L., Wulff, N. A., Pedreira de Miranda, M., Lopes, S. A., Bouwmeester, H., & Schuurink, R. C. (2025). Identification and functional analysis of putative effector proteins from Diaphorina citri and 'Candidatus Liberibacter asiaticus'. Frontiers in Plant Science, 16, Article 1656652. https://doi.org/10.3389/fpls.2025.1656652 -
Kramer, G., de Winde, J. H., Ongenae, V. M., Arentshorst, M., Zwerus, J., Meuken, G., Ijadpanahsaravi, M., Roseboom, W., Ram, A. F. J., Seekles, S. J., van den Brule, T., Punt, M., Dijksterhuis, J., Ohm, R. A., & Wösten, H. A. (2024). Additional file 11 of Compatible solutes determine the heat resistance of conidia [Data set]. Figshare. https://doi.org/10.6084/m9.figshare.26639391.v1
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Kramer, G., de Winde, J. H., Roseboom, W., Arentshorst, M., Ongenae, V. M., Meuken, G., Ijadpanahsaravi, M., Zwerus, J., Ram, A. F. J., Seekles, S. J., van den Brule, T., Punt, M., Dijksterhuis, J., Ohm, R. A., & Wösten, H. A. (2024). Additional file 9 of Compatible solutes determine the heat resistance of conidia [Data set]. Figshare. https://doi.org/10.6084/m9.figshare.26639385.v1
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van Kleeff, P. J. M., Mastop, M., Sun, P., Dangol, S., van Doore, E., Dekker, H. L., Kramer, G., Lee, S., Ryu, C.-M., de Vos, M., & Schuurink, R. (2024). Discovery of three Bemisia tabaci effectors and their effect on gene expression in planta. Molecular Plant-Microbe Interactions, 37(4), 380-395. https://doi.org/10.1094/MPMI-04-23-0044-R -
Šimkovicová, M., Kramer, G., Rep, M., & Takken, F. L. W. (2024). Tomato R-gene-mediated resistance against Fusarium wilt originates in roots and extends to shoots via xylem to limit pathogen colonization. Frontiers in Plant Science, 15, Article 1384431. https://doi.org/10.3389/fpls.2024.1384431
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