mScarlet3 a brilliant and fast-maturing red fluorescent protein
| Authors |
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|---|---|
| Publication date | 04-2023 |
| Journal | Nature Methods |
| Volume | Issue number | 20 | 4 |
| Pages (from-to) | 541-545 |
| Organisations |
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| Abstract | We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems. |
| Document type | Article |
| Note | With Supplementary information |
| Language | English |
| Published at |
https://doi.org/10.1038/s41592-023-01809-y
(Final published version)
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