| Authors |
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| Publication date |
2015
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| Host editors |
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| Book title |
Advanced Fluorescence Microscopy: Methods and Protocols
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| ISBN |
|
| Series |
Methods in Molecular Biology, 1251
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| Pages (from-to) |
135-150
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| Number of pages |
294
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| Publisher |
New York: Humana Press
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| Organisations |
-
Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
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| Abstract |
Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.
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| Document type |
Chapter
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| Language |
English
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| Published at |
https://doi.org/10.1007/978-1-4939-2080-8_8
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