Water-Soluble Anthraquinone Photocatalysts Enable Methanol-Driven Enzymatic Halogenation and Hydroxylation Reactions

Peroxyzymes simply use H₂O₂ as a cosubstrate to oxidize a broad range of inert C-H bonds. The lability of many peroxyzymes against H₂O₂ can be addressed by a controlled supply of H₂O₂, ideally in situ. Here, we report a simple, robust, and water-soluble anthraquinone sulfonate (SAS) as a promising organo-photocatalyst to drive both haloperoxidase-catalyzed halogenation and peroxygenase-catalyzed oxyfunctionalization reactions. Simple alcohols, methanol in particular, can be used both as a cosolvent and an electron donor for H₂O₂ generation. Very promising turnover numbers for the biocatalysts of up to 318 000 have been achieved.