Molecular tools for the diagnosis of malaria and monitoring of parasite dynamics under drug pressure

Thesis

Cover

Titlepage

Contents

Chapter 1: General introduction

Chapter 2: Detection and identification of human Plasmodium species with real-time quantitative nucleic acid sequence-based amplification (Real Time QT-NASBA)

Chapter 3: Development of a Pvs25 based real-time QT-NASBA for the detection of the sexual stage of Plasmodium vivax

Chapter 4: Plasmodium falciparum: evaluation of QT-NASBA to predict the outcome of sulfadoxine-pyrimethamine treatment of uncomplicated malaria

Chapter 5: A randomized trial to monitor efficacy and effectiveness by QT-NASBA of artemether-lumefantrine versus dihydroartemisinin-piperaquine for treatment and transmission control of uncomplicated Plasmodium falciparum malaria in western Kenya

Chapter 6: Molecular biology the best alternative for diagnosis of malaria to microscopy? A comparison between microscopy, antigen detection and molecular tests in rural Kenya and urban Tanzania

Chapter 7: Molecular diagnosis of malaria in the field: development of a novel, nucleic acid lateral flow immuno assay for the detection of all 4 human Plasmodium species and its evaluation in Mbita, Kenya

Chapter 8: General discussion

Summary

Samenvatting

Acknowledgments

Curriculum vitae

List of publications