mScarlet: a bright monomeric red fluorescent protein for cellular imaging
| Authors |
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| Publication date | 01-2017 |
| Journal | Nature Methods |
| Volume | Issue number | 14 | 1 |
| Pages (from-to) | 53-56 |
| Organisations |
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| Abstract | We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging. |
| Document type | Article |
| Language | English |
| Related dataset | Structure of the Red Fluorescent Protein mScarlet at pH 7.8 |
| Published at |
https://doi.org/10.1038/nmeth.4074
(Final published version)
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