Towards application of a human liver cell line for use in the AMC bioartificial liver

Thesis

Cover

Title pages

Contents

Chapter 1: General introduction and outline of the thesis

Part 1: Introduction to the AMC-BAL. Chapter 2: Liver support therapy: an overview of the AMC bioartificial liver research

Chapter 3: Assessment and improvement of liver specific function of the AMC bioartificial liver

Chapter 4: In vitro comparison of two bioartificial liver support systems: MELS CellModule and AMC-BAL

Chapter 5: Mild hypothermic preservation for transport purposes of the AMC bioartificial liver charged with porcine hepatocytes

Part 2: Modeling of the AMC-BAL. Chapter 6: Three-dimensional numerical modeling and Computional Fluid Dynamics (CFD) simulations to analyze and improve oxygen availability in the AMC-BAL

Chapter 7: Enhanced oxygen availability improves liver-specific functions of the AMC bioartificial liver

Chapter 8: Numerical simulation of fluid flow and oxygen transport in a full scale model of the AMC bioartificial liver with an invitro determined hepatocyte distribution

Part 3: A view inside the AMC-BAL: Chapter 9: Time-related analysis of metabolic liver functions, cellular morphology and gene expression of hepatocytes cultured in a bioartificial liver

Chapter 10: Expression of glutamine synthetase and carbamoylphosphate synthetase I in a bioartificial liver: markers for the development of zonation in vitro

Chapter 11: functional and morphological comparison of three primary liver cell types cultured in the AMC bioartificial liver

Part 4: Human liver cell line and the AMC-BAL. Chapter 12: In vitro evaluation of a new immortalized human fetal liver cell line - cBAL111 - for bioartificial liver application

Chapter 13: hepatic encephalopaty and artificial liver support systems

Chapter 14: Evaluation of a novel human fetal liver cell line - CBAL111 - in the AMC-BAL in rats with complete liver ischemia

Chapter 15: Summary of the thesis - Samenvatting van het proefschrift

Part 5: Appendices (contents)

Appendix 1: thoughts and recommendations on chapter 14

Appendix 2: Color images

Appendix 3: abbreviations

Appendix 4: Contributing authors

Appendix 5: Dankwoord/Acknowledgements

Appendix 6: Curriculum vitae

Appendix 7: bibliography

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