RNAi based gene therapy for HIV-1, from bench to bedside

Thesis

Cover

Title pages

Contents

1: Introduction

2: HIV-1 escape is restricted when conserved genome sequences are targeted by RNA interference

3: Probing the sequence space available for HIV-1 evolution

4: Early versus late viral escape upon anti-HIV-1 shRNA pressure

5: Stringent testing identifies highly potent and escape-proof anti-HIV shRNAs

6: Engineering and optimization of the mir-106b-cluster for ectopic expression of multiplexed anti-HIV RNAs

7: Intracellular processing of a polymerase III transcribed RNA with multiple small interfering RNA triggers for RNA interference

8: Viral escape analysis is an effective tool to assess the selection pressure imposed by multiple inhibitors in a combinatorial RNAi therapy

9: Evaluation of safety and efficacy of RNAi against HIV-1 in the human immune system (Rag-2-/-ɣc-/-) mouse model

10: Lentiviral-mediated gene transfer of anti-HIV shRNAs in humanized mice delays thymopoiesis and affects cell accumulation in the peripheral lymphoid organs

11: RNAi based gene therapy for HIV-1 : tackling the hurdles from bench to bedside

Summary

Samenvatting

About the author

List of publications

Acknowledgements