Effect of Vanadium chloroperoxidase on Enterococcus faecalis biofilms

Introduction
The aim of this study was to explore the antimicrobial effect of vanadiumchloroperoxidase (VCPO) reaction products on Enterococcusfaecalisbiofilms of 4 different strains.

Methods
Twenty-four-hour biofilms of E. faecalis strains V583, ER5/1, E2, and OS-16 were incubated in mixtures with VCPO, halide (either bromide or chloride), and hydrogen peroxide. The antibacterial efficacy was assessed by colony-forming unit counts.

Results
The VCPO reaction products had a similar efficacy in reducing the viability of the 4 strains of E. faecalis (94%; range, 87%-100%). Bromide as the halogen of choice was more effective on E. faecalis strains E2 and OS-16, as compared with chloride (Mann-Whitney U test; P < .05). Despite different quantities of produced biofilms by the 4 strains, VCPO treatment was similarly effective toward all strains (Kruskal-Wallis test; P < .05).

Conclusions
VCPO treatment results in an antimicrobial effect toward in vitro E. faecalisbiofilms and might provide an addition to current endodontic treatment, possibly as an antimicrobial dressing.