Micropillar array columns for advancing nanoflow HPLC

HPLC has evolved since the first introduction of instruments, columns, and development of methods and applications. This evolution has culminated in ultra-high performance liquid chromatography (UHPLC) nowadays. However, one may question whether the current separation performance of UHPLC columns has reached the theoretical limit. Reasons for the limitation of the performance of UHPLC and open-tubular liquid chromatography will be presented. Eddy dispersion is the fundamental barrier for further improvement of efficiency; open tubular HPLC theoretically delivers higher separation efficiency but is challenging to use and unavailable commercially. This manuscript aims to describe a new microstructured HPLC column development, characterization of its performance, and compare with standard nanoHPLC packed column technology. Benchmark application and work with samples from life science R&D will be described.