Multi-omics analyses of the molecular physiology and biotechnology of Escherichia coli and Synechocystis sp. PCC6803

The main aim of this thesis was to investigate the physiological response of bacteria responding to physiological- and engineered changes in their central carbon metabolism through the application of multi-omics analyses techniques. The level of the proteome, which is the key functional component of biological systems, and its relationship with its corresponding transcriptome, are the focus of this research. The results described in this thesis have revealed an additional significant layer of regulation of the Carbon Catabolite Repression (CCR) response (i.e. via small, regulatory RNAs) in Escherichia coli. In addition, a number of physiological- and stress responses, caused by overproduction of chemical commodities in Synechocystis cell factories, have been uncovered.