siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Authors
  • M. Raspe
  • K.M. Kedziora
  • B. van den Broek
  • Q. Zhao
Publication date 2016
Journal Nature Methods
Volume | Issue number 13 | 6
Pages (from-to) 501-504
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees -phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
Document type Article
Note With supplementary information
Language English
Published at
https://doi.org/10.1038/NMETH.3836 (Final published version)
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