Immersion oil for high-resolution live-cell imaging at 37°C: Optical and physical characteristics

Authors
  • L.C.J.M. Oomen
  • R. Sacher
  • H.H.J. Brocks
  • J.M. Zwier
Publication date 2008
Journal Journal of Microscopy
Volume | Issue number 232 | 2
Pages (from-to) 353-361
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts.
Document type Article
Language English
Published at https://doi.org/10.1111/j.1365-2818.2008.02106.x
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