Development of an in vitro method to estimate the sensitization induction level of contact allergens
| Authors |
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| Publication date | 02-2018 |
| Journal | Current protocols in toxicology |
| Volume | Issue number | 75 | 1 |
| Pages (from-to) | 20.15.1-20.15.20 |
| Organisations |
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| Abstract | Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE). |
| Document type | Article |
| Language | English |
| Published at |
https://doi.org/10.1002/cptx.44
(Final published version)
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