Spatio-temporal organization of the lipid II flippase MurJ and the lateral wall synthesizing proteins RodA and PBP2

Peptidoglycan is a key component of bacteria cell wall, and plays essential roles for bacteria survive. Thus, its biosynthesis is the main target of many antibiotics. Over years, the abuse of antibiotics has caused serious resistance issues that threatening our life all over the world, which requires the discovery of novel powerful antibiotics. However, the biosynthesis of peptidoglycan and its regulation are still not yet well understood. In this thesis, using E. coli as a model bacterium, we studied the essential protein MurJ that flipping lipid II across the cytoplasmic membrane and elongasome proteins RodA-PBP2 that synthesize peptidoglycan during length growth, and revealed how they function in vivo.