Lipid affinity beads: from identifying new lipid binding proteins to assessing their binding properties

Authors
Publication date 2013
Host editors
  • T. Munnik
  • I. Heilmann
Book title Plant lipid signaling protocols
ISBN
  • 9781627034005
  • 9781493959273
ISBN (electronic)
  • 9781627034012
Series Methods in Molecular Biology
Pages (from-to) 273-280
Publisher New York: Humana Press
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.
Document type Chapter
Language English
Published at https://doi.org/10.1007/978-1-62703-401-2_25
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