Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

Open Access
Authors
Publication date 20-04-2020
Journal ChemCatChem
Volume | Issue number 12 | 8
Pages (from-to) 2180-2183
Organisations
  • Faculty of Science (FNWI) - Van 't Hoff Institute for Molecular Sciences (HIMS)
Abstract The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and kcat of 75 s-1 were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.
Document type Article
Note With supplementary file
Language English
Published at https://doi.org/10.1002/cctc.201902194
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cctc.201902194 (Final published version)
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