Single cell transcriptomics of neighboring hyphae of Aspergillus niger

Open Access
Authors
  • H.A.B. Wösten
Publication date 2011
Journal Genome Biology
Volume | Issue number 12 | 8
Pages (from-to) R71
Number of pages 12
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
Document type Article
Language English
Published at https://doi.org/10.1186/gb-2011-12-8-r71
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