Serious complications in gene-expression studies with stress perturbation: An example of UV-exposed p53-mutant mouse embryonic fibroblasts

Authors
  • O. Bruning
  • X. Yuan
  • W. Rodenburg
  • W. Bruins
Publication date 2010
Journal Transcription
Volume | Issue number 1 | 3
Pages (from-to) 159-164
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design.
Document type Article
Language English
Published at https://doi.org/10.4161/trns.1.3.13487
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