Development of RNA profiling tools and the implementation in forensic casework

In this thesis we have explored the use of mRNA profiling applications as tools for human body fluids and organ tissue identification of biological material found on crime scenes. The chapters cover the developmental processes and validation aspects of two RT-PCR multiplex assays; one aiming at body fluids and skin, and the second identifying organ tissues. In addition, detailed information is provided on the analysis of mixed RNA profiles, interpretation guidelines necessary for the implementation of RNA profiling in forensic laboratory and the prevalence of specific cell types on public objects.