We have studied in this thesis the antimicrobial function of neutrophils from patients with novel primary immunodeficiencies (PIDs), resulting in a susceptibility to infection. The neutrophils from patients with CARD9 deficiency are impaired in the killing of C. albicans and this is associated with invasive fungal infections. In addition, the antimicrobial function of G-CSF/dexamethasone-mobilized neutrophils used for transfusion purposes was determined. Experiments with these human “knock-out” neutrophils have expanded our knowledge about the role of Pathogen Recognition Receptors and signaling in microbial killing in humans. Neutrophils have distinct mechanisms for the killing of S. aureus, E. coli, C. albicans or A. fumigatus conidia and hyphae. The formation of Neutrophil Extracellular Traps (NETs) is not required for the killing of C. albicans or A. fumigatus. G-CSF/dexamethasone-mobilized neutrophils used for transfusion purposes, are impaired in Candida yeast killing, but normally kill bacterial and fungal pathogens.
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.