siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Raspe, M.; Kedziora, K.M.; van den Broek, B.; Zhao, Q.; de Jong, S.; Herz, J.; Mastop, M.; Goedhart, J.; Gadella, T.W.J.; Young, I.T.; Jalink, K.

doi:http://dx.doi.org/10.1038/NMETH.3836

Author: M. Raspe
K.M. Kedziora
B. van den Broek
Q. Zhao
S. de Jong
J. Herz
M. Mastop
J. Goedhart
T.W.J. Gadella
I.T. Young
K. Jalink
Year: 2016
Title: siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
Journal: Nature Methods
Volume | Issue number: 13 | 6
Pages (from-to): 501-504
Document type: Article
Faculty: Faculty of Science (FNWI)
Institute: Swammerdam Institute for Life Sciences (SILS)
Abstract: We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees -phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
URL: go to publisher's site
Link: Link
Language: English
Note: With supplementary information
Permalink: http://hdl.handle.net/11245/1.535309

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