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Author
M. Raspe
K.M. Kedziora
B. van den Broek
Q. Zhao
S. de Jong
J. Herz
M. Mastop
J. Goedhart
T.W.J. Gadella
I.T. Young
K. Jalink
Year
2016
Title
siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
Journal
Nature Methods
Volume | Issue number
13 | 6
Pages (from-to)
501-504
Document type
Article
Faculty
Faculty of Science (FNWI)
Institute
Swammerdam Institute for Life Sciences (SILS)
Abstract
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees -phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
URL
go to publisher's site
Link
Link
Language
English
Note
With supplementary information
Permalink
http://hdl.handle.net/11245/1.535309

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