- Author
-
M. Raspe
K.M. Kedziora
B. van den Broek
Q. Zhao
S. de Jong
J. Herz
M. Mastop
J. Goedhart
T.W.J. Gadella
I.T. Young
K. Jalink - Year
- 2016
- Title
- siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
- Journal
- Nature Methods
- Volume | Issue number
- 13 | 6
- Pages (from-to)
- 501-504
- Document type
- Article
- Faculty
- Faculty of Science (FNWI)
- Institute
- Swammerdam Institute for Life Sciences (SILS)
- Abstract
- We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees -phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
- URL
- go to publisher's site
- Link
- Link
- Language
- English
- Note
- With supplementary information
- Permalink
- http://hdl.handle.net/11245/1.535309
Disclaimer/Complaints regulations
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.