- Spore Proteomics: The Past, Present and the Future
- FEMS Microbiology Letters
- Volume | Issue number
- 358 | 2
- Pages (from-to)
- Document type
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
Endospores are metabolically dormant, multi-layered cellular structures formed by Gram positive bacteria belonging to the genera Bacillus, Clostridium and related organisms. Their external layers are composed of proteins which in part play a role in resistance behaviour of spores to varied chemical and environmental assaults. Thus, protein analysis is of major interest in spore biology. Spore proteomic studies have been carried out previously but these studies have focussed on the soluble coat protein fraction. Using gel-based techniques protein identification and analysis was performed. Mass spectrometry driven proteomics has opened new avenues to resolve in particular the insoluble part of the spore layer proteomes. Mass spectrometry based qualitative and quantitative proteomics methods expand the knowledge about both the actual composition and the amount of proteins in their various layers. The techniques can also be used to study the integrity of the layers as well as spore biology in general. This notion is explored concisely in this mini-review.
- go to publisher's site
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.