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Author
G. Simons
J.P. Groenendijk
J. Wijbrandi
M. Reijans
J. Groenen
P.J. Diergaarde
T. van der Lee
M. Bleeker
J. Onstenk
Year
1998
Title
Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy
Journal
The Plant Cell
Volume
10
Pages (from-to)
1055-1068
Number of pages
14
Document type
Article
Faculty
Faculty of Science (FNWI)
Institute
Swammerdam Institute for Life Sciences (SILS)
Abstract
The I-2 locus in tomato confers resistance to race 2 of the soil-borne fungus Fusarium oxysporum f sp lycopersici. The selective restriction fragment amplification (AFLP) positional cloning strategy was used to identify I-2 in the tomato genome. A yeast artificial chromosome (YAC) clone covering ~750 kb encompassing the I-2 locus was isolated, and the AFLP technique was used to derive tightly linked AFLP markers from this YAC clone. Genetic complementation analysis in transgenic R1 plants using a set of overlapping cosmids covering the I-2 locus revealed three cosmids giving full resistance to F. o. lycopersici race 2. These cosmids shared a 7-kb DNA fragment containing an open reading frame encoding a protein with similarity to the nucleotide binding site leucine-rich repeat family of resistance genes. At the I-2 locus, we identified six additional homologs that included the recently identified I-2C-1 and I-2C-2 genes. However, cosmids containing the I-2C-1 or I-2C-2 gene could not confer resistance to plants, indicating that these members are not the functional resistance genes. Alignments between the various members of the I-2 gene family revealed two significant variable regions within the leucine-rich repeat region. They consisted of deletions or duplications of one or more leucine-rich repeats. We propose that one or both of these leucine-rich repeats are involved in Fusarium wilt resistance with I-2 specificity.
Language
Undefined/Unknown
Permalink
http://hdl.handle.net/11245/1.427297

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