- Mechanical stimulation of bone cells using fluid flow
- Methods in Molecular Biology
- Pages (from-to)
- Document type
- Faculty of Dentistry (ACTA)
- This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
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