Isolation of primary avian osteocytes

Semeins, C.M.; Bakker, A.D.; Klein-Nulend, J.

doi:http://dx.doi.org/10.1007/978-1-61779-415-5_4

Author: C.M. Semeins
A.D. Bakker
J. Klein-Nulend
Year: 2012
Title: Isolation of primary avian osteocytes
Journal: Methods in Molecular Biology
Volume: 816
Pages (from-to): 43-53
Document type: Article
Faculty: Faculty of Dentistry (ACTA)
Abstract: Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
URL: go to publisher's site
Language: English
Permalink: http://hdl.handle.net/11245/1.372724

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