A.M. van Kuijen
- Monitoring microcirculatory alterations in oral squamous cell carcinoma following photodynamic therapy
- Photodiagnosis and Photodynamic Therapy
- Volume | Issue number
- 9 | 1
- Pages (from-to)
- Document type
- Faculty of Dentistry (ACTA)
Faculty of Medicine (AMC-UvA)
One of the mechanisms through which photodynamic therapy (PDT) is thought to elicit tumour destruction is by producing microvascular damage and obstruction of nutritive blood flow. The aim of this study was to directly monitor and quantify microcirculatory changes following tissue illumination by PDT for oral squamous cell carcinoma.
Ten consecutive patients receiving PDT for a carcinoma in situ, a T1 or T2 tumour in the oral cavity without evidence of lymph node metastasis were selected for this study. Tumour and marginal healthy mucosa total capillary density (TCD) and functional capillary density (FCD) inside the field of illumination were measured and compared using sidestream dark-field (SDF) imaging prior to tissue illumination, immediately after PDT, and again after 15 min.
Baseline mean tumour TCD was 21.2 ± 5 capillaries per square millimetres (cpll/mm2) and 24.9 ± 19 cpll/mm2 in the surrounding marginal healthy tissue; there were no significant differences between tumour and healthy tissue or time points. Comparisons between baseline and post-illumination time points revealed significant differences in both tumour and healthy tissue FCD (P < 0.05). No significant differences in FCD were observed between the two tissues.
Our findings using SDF imaging demonstrate that PDT significantly attenuates tumour and marginal healthy tissue perfusion by directly disrupting the functionality of the microcirculation.
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