- Dual compartment neurofluidic system for electrophysiological measurements in physically isolated neuronal cell cultures
- Book/source title
- EMBC 2009
- Book/source subtitle
- proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society : "engineering the future of biomedicine" : 2-6 September, 2009, Hilton Minneapolis, Minnesota
- Pages (from-to)
- Piscataway, NJ: IEEE
- ISBN (electronic)
- Document type
- Conference contribution
- Faculty of Social and Behavioural Sciences (FMG)
- Psychology Research Institute (PsyRes)
This work investigates an approach to record electrophysiological measurements of neuronal cell cultures in a dual compartment neurofluidic system. The two compartments are separated by 10-μm-wide and 3-μm-high microchannels and this provides a physical isolation of neurons allowing only neurites to grow between the compartments. We
present long-term cell viability in closed compartment devices, neurite growth across the microchannels and a recording setup for the long-term recording of the network activity over 21 Days-in-Vitro (DIV). Structural and fluidic isolation between the compartments are demonstrated using transfection experiments and neurotoxin exposure, respectively.
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