- Dual compartment neurofluidic system for electrophysiological measurements in physically isolated neuronal cell cultures
- Book/source title
- 31st Annual International Conference of the IEEE EMBS
- Pages (from-to)
- Minneapolis, Minnesota, USA
- Document type
- Conference contribution
- Faculty of Social and Behavioural Sciences (FMG)
- Psychology Research Institute (PsyRes)
This work investigates an approach to record electrophysiological measurements of neuronal cell cultures in a dual compartment neurofluidic system. The two compartments are separated by 10-μm-wide and 3-μm-high microchannels and this provides a physical isolation of neurons allowing only neurites to grow between the compartments. We
present long-term cell viability in closed compartment devices, neurite growth across the microchannels and a recording setup for the long-term recording of the network activity over 21 Days-in-Vitro (DIV). Structural and fluidic isolation between the compartments are demonstrated using transfection experiments and neurotoxin exposure, respectively.
- go to publisher's site
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.