- Maximizing the peak production rate in off-line comprehensive two-dimensional liquid chromatography with mass spectrometry detection
- LC GC Europe
- Volume | Issue number
- 22 | 8
- Pages (from-to)
- Document type
- Faculty of Science (FNWI)
- Van 't Hoff Institute for Molecular Sciences (HIMS)
This article describes an optimization strategy to obtain the best possible performance in the shortest analysis time—called the peak production rate—for comprehensive off-line two-dimensional liquid chromatography. The demands on column technology (particle size and column length) and LC conditions (including sampling time, gradient time and slope, and column-equilibration time) are discussed. The potential of off-line 2D-LC coupled to tandem mass spectrometry detection is demonstrated with the analysis of proteomics samples of various complexities and a trade-off is made between the 1D-LC and off-line 2D-LC performance.
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