An innovative method to study target protein-drug interactions by mass spectrometry

Müller, M.Q.; de Koning, L.J.; Schmidt, A.; Ihling, A.; Syha, Y.; Rau, O.; Mechtler, K.; Schubert-Zsilavecz, M.; Sinz, A.

doi:http://dx.doi.org/10.1021/jm9000665

Author: M.Q. Müller
L.J. de Koning
A. Schmidt
A. Ihling
Y. Syha
O. Rau
K. Mechtler
M. Schubert-Zsilavecz
A. Sinz
Year: 2009
Title: An innovative method to study target protein-drug interactions by mass spectrometry
Journal: Journal of Medicinal Chemistry
Volume | Issue number: 52 | 9
Pages (from-to): 2875-2879
Document type: Article
Faculty: Faculty of Science (FNWI)
Institute: Swammerdam Institute for Life Sciences (SILS)
Abstract: We report the combination of chemical cross-linking and high-resolution mass spectrometry for analyzing conformational changes in target proteins that are induced by drug binding. With this approach conformational changes in the peroxisome proliferator-activated receptor alpha (PPARα) upon binding of low-molecular weight compounds were readily detected, proving that the strategy provides a basis to efficiently characterize target protein−drug interactions.
URL: go to publisher's site
Language: Undefined/Unknown
Permalink: http://hdl.handle.net/11245/1.313084

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