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Author
G.L. Torres de Heens
R. Kikkert
L.A. Aarden
U. van der Velden
B.G. Loos
Year
2009
Title
Effects of smoking on the ex vivo cytokine production in periodontitis
Journal
Journal of Periodontal Research
Volume | Issue number
44 | 1
Pages (from-to)
28-34
Document type
Article
Faculty
Faculty of Medicine (AMC-UvA)
Faculty of Dentistry (ACTA)
Abstract
Background and Objective:  Smoking is associated with increased severity of periodontitis. The underlying mechanisms of this phenomenon are not well understood. The purpose of the present study was to compare the monocyte-derived T cell directing (Th1/Th2) response and pro-inflammatory cytokine production in ex vivo whole blood cell cultures (WBCC) of smoking and non-smoking chronic periodontitis patients.
Material and Methods:  Venous blood was collected from 29 periodontitis patients (18 non-smokers and 11 smokers) receiving supportive periodontal treatment, and diluted 10-fold for WBCC. The WBCC were stimulated for 18 h with Neisseria meningitidis lipo-oligosaccharide (LOS) orPorphyromonas gingivalis sonic extract (Pg-SE). The production of the T cell directing cytokines interleukin (IL)-12 p40 and IL-10, as well as the pro-inflammatory cytokines IL-1β, IL-6 and IL-8, was measured in the culture supernatants.
Results:  After LOS stimulation of WBCC, smokers showed a lower IL-12 p40/IL-10 ratio than non-smokers (P < 0.05). Interleukin-1β production was significantly lower in smokers compared with non-smokers after stimulation with either LOS or Pg-SE (P < 0.05). Interleukin-6 and IL-8 production was similar in WBCC from both smokers and non-smokers, for both LOS and Pg-SE.
Conclusion:  A more pronounced Th2 response in smoking periodontitis patients may be related to increased severity of the disease.
URL
go to publisher's site
Language
Undefined/Unknown
Permalink
http://hdl.handle.net/11245/1.306964

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