- Enzymatic and chemical degradation of curdlan targeting the production of β-(1 → 3) oligoglucans
- Carbohydrate polymers
- Volume | Issue number
- 71 | 2
- Pages (from-to)
- Document type
- Faculty of Science (FNWI)
- Van 't Hoff Institute for Molecular Sciences (HIMS)
Obtaining biologically active oligosaccharides from hydrolysed polysaccharides is nowadays of great interest. Curdlan is one of the polysaccharides with interesting properties and represents the main source for β-(1 → 3)-oligoglucans. Curdlan degradation remains a challenge due to its high resistance to hydrolysis. In this study, curdlan was hydrolysed both with mild acid treatments (TFA or H2SO4, 1 M) as well as with the use of an unpurified enzymic extract produced from Trichoderma harzianum. The kinetics of degradation and the production of the β-(1 → 3)-oligoglucans were followed in detail by HPAEC analysis. A comparison of results clearly demonstrated the advantage of the enzymic extract with a complete hydrolysis of the curdlan within 90 h whereas less than 25% hydrolysis yield can be reach with the acidic methodologies. More, the quality of the obtained compounds is particularly dissimilar even between the two acid treatments.
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