- CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome
- International immunology
- Volume | Issue number
- 18 | 11
- Pages (from-to)
- Document type
- Faculty of Science (FNWI)
Faculty of Medicine (AMC-UvA)
- Swammerdam Institute for Life Sciences (SILS)
The development of a strong T(h)1-mediated adaptive immune response is considered of main importance for host defense against the intracellular pathogen Mycobacterium tuberculosis. The induction of a cellular immune response is not only dependent on the engagement of the TCR but also requires co-stimulation. In order to study the role of the co-stimulatory molecule of the tumor necrosis factor receptor family member CD27 during murine M. tuberculosis infection, we intranasally infected wild-type (WT) and CD27 knockout (KO) mice with 10(5) colony-forming units M. tuberculosis. Whereas there were no differences in bacterial growth, inflammation and IFN gamma production by CD4(+) and CD8(+) lymphocytes in the lungs early after infection, the number of splenic CD8(+) T cells producing the key T(h)1 cytokine IFN gamma was lower in CD27 KO mice than in WT mice. After 6 weeks, CD27 KO mice had 3.6-fold higher mycobacterial counts in their lungs and displayed more pulmonary inflammation and increased numbers of infiltrated leukocytes. Despite these differences early in infection, an equal number of WT and CD27 KO mice died during a 43-week observation period and lung bacterial loads and inflammation were comparable in the surviving animals. Our data suggest that CD27 does not contribute to the local IFN gamma-mediated response and long-term protection against M. tuberculosis.
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