14-3-3 proteins form a family of highly conserved eukaryotic proteins involved in a wide variety of cellular processes, including
signalling, apoptosis, cell-cycle control and transcriptional regulation. More than 150 binding partners have been found for
these proteins. The yeast Saccharomyces cerevisiae has two genes encoding 14-3-3 proteins, BMH1 and BMH2. A bmh1 bmh2 double
mutant is unviable in most laboratory strains. Previously, we constructed a temperature-sensitive bmh2 mutant and showed that
mutations in RTG3 and SIN4, both encoding transcriptional regulators, can suppress the temperature-sensitive phenotype of
mutant, suggesting an inhibitory role of the 14-3-3 proteins in Rtg3-dependent transcription [van Heusden and Steensma
(2001) Yeast 18, 1479¿1491]. In the present paper, we report a genomewide transcription analysis of a temperature-sensitive
bmh2 mutant. Steady-statemRNAlevels of 60 open reading frameswere increased more than 2.0-fold in the bmh2 mutant, whereas
those of 78 open reading frames were decreased more than 2.0-fold. In
agreement with our genetic experiments, six genes
known to be regulated by Rtg3 showed elevated mRNA levels in the mutant.
In addition, several genes with other cellular
functions, including those involved in gluconeogenesis, ergosterol biosynthesis and stress response, had altered mRNA levels
in the mutant. Our data show that the yeast 14-3-3 proteins negatively regulate Rtg3- dependent transcription, stimulate the
transcription of genes involved in ergosterol metabolism and in stress response and are involved in transcription regulation
of multiple other genes.