- A new method for mite extraction from leaf samples
- Experimental and Applied Acarology
- Volume | Issue number
- 32 | 1-2
- Number of pages
- Document type
- Faculty of Science (FNWI)
- Institute for Biodiversity and Ecosystem Dynamics (IBED)
Debris often hampers the detection of mites in washed leaf samples. We describe in detail a method for the extraction of mites from leaf samples, based on the adherence of mite cuticles to liquid paraffin, at the interface of paraffin and ethanol in a so-called mite-counting channel. We demonstrate its efficacy by comparing the mite numbers in samples before and after extraction. We illustrate the method's reliability by extracting known numbers of a taxonomic variety of plant-inhabiting mites, manually added to mite-free debris: for 13 of the 15 taxa all mites were retrieved. This method can also be used to extract small non-mite arthropods such as scales, whiteflies, thrips, cicadellids, hymenopteran parasitoids and psyllids.
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