- Affinities of the Aphanocapsa feldmanni-like cyanobacteria from the marine sponge Xestospongia muta based on genetic and morphological analyses
- Bolletino di Museo e Istituto di Biologia dell'Universita di Genova
- Number of pages
- Document type
- Faculty of Science (FNWI)
- Institute for Biodiversity and Ecosystem Dynamics (IBED)
The marine sponge Xestospongia muta (Porifera: Demospongiae: Haplosclerida) harbours cyanobacteria in its peripheral tissue that have been described as having an Aphanocapsa feldmanni-type appearance. Through subsequent cell fractionation steps we obtained a virtually pure cell suspension of the cyanobacteria. Through amplification of a region of the 16S rRNA gene we found that these cyanobacteria seem closely related to Prochlorococcus (Cyanobacteria, Prochlorophytes, Prochlorococcaceae), and Synechococcus (Cyanobacteria, Chroococcales, Synechococcus). We furthermore developed a new method, to obtain a clear signal with FISH by bleaching the
auto-fluorescence of cyanobacteria, with osmium tetroxide. The location and morphological characteristics of the cyanobacteria are described by both light and electron microscopy.
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.