- Direct measurement of chain order in single lipid mono- and bilayers with multiplex CARS.
- The journal of Physical Chemistry. B
- Number of pages
- Document type
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
We present direct measurements of the thermodynamic state of single phospholipid mono- and bilayers based on the intrinsic vibrational properties of the molecules. The increased, shot-noise limited, sensitivity of multiplex coherent anti-Stokes Raman scattering permits the acquisition of vibrational spectra of single lipid bilayers with a signal-to-noise ratio of 4 in 20 ms. The lipid chain order for both liquid-crystalline and gel phase supported lipid bilayers is determined. Using deuterated lipids, vibrational spectra can be measured selectively in either of the leaflets of an asymmetric bilayer.
- go to publisher's site
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.