- Shot-noise limited detection sensitivity in multiplex CARS microscopy.
- Number of pages
- Document type
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
In multiplex CARS microscopy the generated anti-Stokes signal is generated and detected simultaneouslyover a significant part of the vibrational spectrum. The signal-to-noise ratio of the thus detected spectra islimited only by shot-noise. This principle is demonstrated using a dilution series of 2-propanol in water.It is derived theoretically and shown experimentally that for low solute concentrations - in contrast tomethods that suppress the non-resonant background - the CARS signal strength from a particularvibrational mode depends linearly on its concentration. Furthermore, excellent agreement is shownbetween the experimental data and fits to the theory. It is shown that this approach permits rapid (20 msacquisition) detection of a single lipid mono-layer, with sufficient signal-to-noise to determine the orderparameter for the acyl chain packing. Also it is demonstrated that this detection scheme provides anabsolute measure of the solute concentration.
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