- Molecular and biochemical studies of fragrance biosynthesis in rose
- Award date
- 17 March 2017
- Number of pages
- Document type
- PhD thesis
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
Roses are one of the most popular ornamental plants, whose floral volatiles are not only involved in environmental interactions but also widely used by industries. The biosynthesis of many of these volatiles in roses is not well understood. This thesis describes alternative pathways for the biosynthesis of volatile terpenoids in rose petals, especially geraniol and E,E-farnesol. Their production occurs via the hydrolysis of prenyl diphosphates by Nudix hydrolase 1 (NUDX1) enzymes of which two members are characterized. In a quest to identify the transcription factors regulating the expression of the NUDX1 genes and to identify other scent-related genes, transcriptomes of scented and unscented roses were obtained and analyzed leading to several candidates.
French title of the thesis: Etude de gènes impliqués dans la biosynthèse du parfum chez la rose, Rosa x hybrida.
- Joint doctorate degree awarded by the University of Amsterdam and Université Jean Monnet Saint-Étienne.
Thesis (complete) (Embargo up to and including 17 March 2019)
Chapter 3: Nudix hydrolase 1 proteins are key factors of the biosynthesis of both monoterpenoids and sesquiterpenoids in roses (Embargo up to and including 17 March 2019)
Chapter 4: Transcriptional regulation of Nudix hydrolase 1 in roses (Embargo up to and including 17 March 2019)
Chapter 5: De novo transcriptome assembly and RNA-Seq analysis for rose petals to discover scent-related genes and transcription factors (Embargo up to and including 17 March 2019)
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