S. del Prete
- Characterisation of transcriptional and chromatin events in relation to floral transition and identification of nuclear organisation determinants
- Award date
- 21 March 2017
- Number of pages
- Document type
- PhD thesis
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
The transition to flowering results from a complex interplay between endogenous and environmental cues. The leaves play a key role in this process, by perceiving the light changes and producing photosynthates, which participate to the floral signalling. However, our knowledge on the changes occurring in leaves during floral transition is still limited. We characterised the morphological, molecular and transcriptional events related to floral transition in mature leaves in Arabidopsis, using a short-day to long-day shift to induce a synchronized flowering. We identified the temporal window of the floral transition, monitored the leaf growth and observed an increase in their ploidy level during the process. By RNA-seq we studied the transcriptional dynamics of the leaf gene network, and compared with events occurring in roots and meristems to get an integrated view of floral transition in the whole plant. Furthermore, we investigated the mode of action of LIKE HETEROPROTEIN 1 (LHP1), a PRC1 subunit, by exploiting transgenic lines with conditional alterations of LHP1 dosage and analysing the effects on chromatin and transcription of flowering genes. A short-term modulation of LHP1 dosage altered the deposition of H3K27me3 and H3K4me3, showing a functional interaction between LHP1 and PRC2, and also suggesting a new role in the formation of bivalent chromatin regions. Finally, since nuclear organisation plays a key role in gene regulation, we searched and identified determinants of the nuclear architecture by using innovative spatial statistical tools.
- This thesis was prepared within the partnership between the University of Amsterdam and University Paris Saclay with the purpose of obtaining a joint doctorate degree.
Thesis (complete) (Embargo up to and including 21 March 2019)
CHAPTER 1: Nuclear architecture and chromatin dynamics in interphase nuclei of Arabidopsis thaliana (Embargo up to and including 21 March 2019)
CHAPTER 2: One, two, three: Polycomb proteins hit all dimensions of gene regulation (Embargo up to and including 21 March 2019)
CHAPTER 3: Molecular and morphological characterisation of the floral transition in Arabidopsis thaliana induced by photoperiodic switch (Embargo up to and including 21 March 2019)
CHAPTER 4: Early transcriptional events associated with floral transition in Arabidopsis leaves induced by a short-day to long-day switch (Embargo up to and including 21 March 2019)
CHAPTER 5: Alteration of LHP1 dosage and its impact on gene regulation and chromatin marks (Embargo up to and including 21 March 2019)
CHAPTER 6: Molecular determinants of the 3D nuclear organization in Arabidopsis (Embargo up to and including 21 March 2019)
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