- Trade-offs to win-win
- Smart-fluorescence imaging for improved image quality with reduced photodamage
C.J.F. van Noorden
- Award date
- 22 November 2017
- Number of pages
- Document type
- PhD thesis
- Faculty of Science (FNWI)
- Swammerdam Institute for Life Sciences (SILS)
Bio-imaging using fluorescence microscopy is widely used for the study and visualization of biological processes with a high degree of specificity. However, the illumination used for fluorescence imaging can be invasive enough to affect the imaging process by causing photodamage. At the same time, the quality of the recorded image largely depends on the illumination levels used for imaging resulting in a trade-off between image quality and photodamage. In this study smart illumination strategies are explored to reduce photodamage and improve imaging sensitivity. The study concludes that by adjusting illumination in accordance to the spatial-profile of the biological specimen during real-time imaging, it is possible to improve image quality and reduce photodamage at the same time leading to a win-win scenario.
Thesis (complete) (Embargo up to and including 22 November 2019)
Chapter 4: Ultra-sensitive fluorescence imaging with spatially-controlled illumination in rescan confocal microscopy reduces photodamage (Embargo up to and including 22 November 2019)
Chapter 5: On why spatial-control of illumination is a good idea for fluorescence microscopy (Embargo up to and including 22 November 2019)
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