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| Authors | J. Muris, C.J. Kleverlaan, A.J. Feilzer, E. Valentine-Thon | | Title | Reactivity to sodium tetrachloropalladate (Na-2[PdCl4]) compared to PdCl2 and NiCl2 in lymphocyte proliferation tests |
| Journal | Allergy |
| Volume | 64 |
| Year | 2009 |
| Issue | 8 |
| Pages | 1152-1156 |
| ISSN | 01054538 |
| Faculty | ACTA |
| Abstract | Background: For patch testing, replacement of the commonly used palladium dichloride (PdCl2) by sodium tetrachloropalladate (Na2[PdCl4]) was recently demonstrated to improve test accuracy and show a significant correlation with nickel (Ni), supporting the concept of cross-reactivity between Pd and Ni. A promising alternative to metal allergy patch testing is the in vitro lymphocyte proliferation test (LTT).
Objectives: The aim of this study was to test whether Na2[PdCl4] is also more sensitive for diagnosing Pd allergy with a standardized LTT.
Patients/methods: After determining optimal nontoxic and nonmitogenic concentrations for Na2[PdCl4], blood samples from 105 patients with clinical suspicion of metal allergy were tested with an LTT called memory lymphocyte immuno stimulation assay for Na2[PdCl4], PdCl2 and NiCl2. Reaction profiles were analysed for concordant positive reactions.
Results: Using the conventional cut-off of stimulation index ≥ 3, 74.3% showed a positive reaction to NiCl2, 15.2% to PdCl2 and 28.6% to Na2[PdCl4]. All positive results to PdCl2 were covered by Na2[PdCl4]. From the 30 positive reactions to Na2[PdCl4], 26 (87%) were concordant for NiCl2 reactivity.
Conclusion: In LTT, the use of Na2[PdCl4] results in more positive reactions in Pd allergy testing which are in concordance with positive reactions to PdCl2 and NiCl2. |
| Document type | Article |
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