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| Auteurs | M.M. Julkowska, F. McLoughlin, C.S. Galvan-Ampudia, J.M. Rankenberg, D. Kawa, M. Klimecka, M.A. Haring, T. Munnik, E.E. Kooijman, C. Testerink | | Titel | Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain. |
| Tijdschrift | Plant, cell and environment |
| Jaargang | 38 |
| Jaar | 2015 |
| Nummer | 3 |
| Pagina's | 614-624 |
| ISSN | 01407791 |
| Faculteit | Faculteit der Natuurwetenschappen, Wiskunde en Informatica |
| Instituut/afd. | FNWI: Swammerdam Institute for Life Sciences (SILS) |
| Samenvatting | Phosphatidic acid (PA) is an important signalling lipid involved in various stress-induced signalling cascades. Two SnRK2 protein kinases (SnRK2.4 and SnRK2.10), previously identified as PA-binding proteins, are shown here to prefer binding to PA over other anionic phospholipids and to associate with cellular membranes in response to salt stress in Arabidopsis roots. A 42 amino acid sequence was identified as the primary PA-binding domain (PABD) of SnRK2.4. Unlike the full-length SnRK2.4, neither the PABD-YFP fusion protein nor the SnRK2.10 re-localized into punctate structures upon salt stress treatment, showing that additional domains of the SnRK2.4 protein are required for its re-localization during salt stress. Within the PABD, five basic amino acids, conserved in class 1 SnRK2s, were found to be necessary for PA binding. Remarkably, plants overexpressing the PABD, but not a non-PA-binding mutant version, showed a severe reduction in root growth. Together, this study biochemically characterizes the PA-SnRK2.4 interaction and shows that functionality of the SnRK2.4 PABD affects root development. |
| Taal | Engels |
| Opmerkingen | With supporting information |
| URL | go to publisher's site |
| Soort document | artikel |
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