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Zoekopdracht: faculteit: "ACTA" en publicatiejaar: "2010"

AuteursA.A. Kranendonk, W.A. van der Reijden, A.J. van Winkelhoff, G.A. van der Weijden
TitelThe bactericidal effect of a Genius Nd: YAG laser
TijdschriftInternational Journal of Dental Hygiene
Jaargang8
Jaar2010
Nummer1
Pagina's63-67
ISSN16015029
FaculteitACTA
SamenvattingPurpose: To evaluate the ‘in vitro’ bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens. Methods: Suspensions of six different periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum and Parvimonas micra) were prepared in small Eppendorff tubes, and exposed to a Nd:YAG laser for five different periods of time. Laser settings used: Power 6 Watt (on a scale of 1–12 W), Frequency 50 Hz, Pulse duration 250 μs. After exposure to the laser, aliquots of the suspensions were spread on blood agar plates for bacterial counting. Results: After 5 s of laser exposure, there was a decrease in total colony forming units for all six selected microorganisms. After 15, 30 and 45 s, no viable bacterial cells could be retrieved. Conclusion: In this ‘in vitro’ model, 15 s of Nd:YAG laser use was found to be effective for total killing of the six tested periodontal pathogens.
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