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Zoekresultaten

Zoekopdracht: faculteit: "FNWI" en publicatiejaar: "2012"

AuteursE. Lukasik-Shreepaathy, J.H. Vossen, W.I.L. Tameling, M.J. de Vroomen, B.J.C. Cornelissen, F.L.W. Takken
TitelProtein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2
TijdschriftJ Exp Bot
Jaargang63
Jaar2012
Nummer8
Pagina's3047-3060
ISSN00220957
FaculteitFaculteit der Natuurwetenschappen, Wiskunde en Informatica
Instituut/afd.FNWI: Swammerdam Institute for Life Sciences (SILS)
SamenvattingPlant resistance proteins (R) are involved in pathogen recognition and subsequent initiation of defence responses. Their activity is regulated by inter- and intramolecular interactions. In a yeast two-hybrid screen two clones (I2I-1 and I2I-2) specifically interacting with I-2, a Fusarium oxysporum f. sp. lycopersici resistance protein of the CC-NB-LRR family, were identified. Sequence analysis revealed that I2I-1 belongs to the Formin gene family (SlFormin) whereas I2I-2 has homology to translin-associated protein X (SlTrax). SlFormin required only the N-terminal CC I-2 domain for binding, whereas SlTrax required both I-2 CC and part of the NB-ARC domain. Tomato plants stably silenced for these interactors were not compromised in I-2-mediated disease resistance. When extended or mutated forms of I-2 were used as baits, distinct and often opposite, interaction patterns with the two interactors were observed. These interaction patterns correlated with the proposed activation state of I-2 implying that active and inactive R proteins adopt distinct conformations. It is concluded that the yeast two hybrid system can be used as a proxy to monitor these different conformational states.
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