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Zoekopdracht: faculteit: "FNWI" en publicatiejaar: "1990"

AuteursW.M.R. van den Akker, J. Westerman, Th.W.J. Gadella, K.W.A. Wirtz, G.T. Snoek
TitelProteolytic activation of bovine brain protein with phosphatidylinositol transfer activity
TijdschriftFEBS letters : for the rapid publication of short reports in biochemistry, biophysics and molecular biology
Jaargang276
Jaar1990
Pagina's123-126
ISSN0014-5793
FaculteitFaculteit der Natuurwetenschappen, Wiskunde en Informatica
Instituut/afd.FNWI: Swammerdam Institute for Life Sciences (SILS)
TrefwoordenProtein purification
SamenvattingWe have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylino-sitol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in phosphatidylinositol transfer activity and a IO-fold higher affinity for this phospholipid. The possibility that the 38 kDa protein is a precursor of the phosphatidylinositol transfer protein is discussed. (C) 1990 Elsevier Science B.V. All rights reserved.
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