The UvA-LINKER will give you a range of other options to find the full text of a publication (including a direct link to the full-text if it is located on another database on the internet).
De UvA-LINKER biedt mogelijkheden om een publicatie elders te vinden (inclusief een directe link naar de publicatie online als deze beschikbaar is in een database op het internet).
faculty: "FNWI" and publication year: "2007"
| Authors||T. van Opijnen, A. de Ronde, M.C. Boerlijst, B. Berkhout|
|Title||Adaptation of HIV-1 Depends on the Host-Cell Environment|
|Faculty||Faculty of Science|
|Institute/dept.||FNWI: Institute for Biodiversity and Ecosystem Dynamics (IBED)|
|Abstract||Many viruses have the ability to rapidly develop resistance against antiviral drugs and escape from the host immune system. To which extent the host environment affects this adaptive potential of viruses is largely unknown. Here we show that for HIV-1, the host-cell environment is key to the adaptive potential of the virus. We performed a large-scale selection experiment with two HIV-1 strains in two different T-cell lines (MT4 and C8166). Over 110 days of culture, both virus strains adapted rapidly to the MT4 T-cell line. In contrast, when cultured on the C8166 T-cell line, the same strains did not show any increase in fitness. By sequence analyses and infections with viruses expressing either yellow or cyan fluorescent protein, we were able to show that the absence of adaptation was linked to a lower recombination rate in the C8166 T-cell line. Our findings suggest that if we can manipulate the host-cellular factors that mediate viral evolution, we may be able to significantly retard viral adaptability.|
Use this url to link to this page: http://dare.uva.nl/en/record/287079
Contact us about this recordNotify a colleague
Add to bookbag